 | Smart-Taq DNA Polymerase, 5 U/µl |
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Enzyme propertiesThis thermostable DNA polymerase is purified from recombinant E. coli strain with cloned gene encoding Thermus aquaticus DNA polymerase. Smart-Taq has 5' > 3' DNA synthesis activity. Quality data Activity and stability tested at 20, 30 and 40 cycles of PCR reactions at 95 degrees C. Tested for the absence of human DNA contamination by PCR with Alu-specific primers. Unit definition One unit of the enzyme catalyzes the incorporation of 10 nanomoles of deoxyribonucleotides into a polynucleotide fraction at 70 degrees C. Composition of the set Smart-Taq in storage buffer 10X Reaction buffer 10X Reaction buffer with ammonium sulfate 25 mM Magnesium chloride solution Storage conditions Smart-Taq will remain stable at -20 degrees C in a constant temperature freezer. |